An Improved Method for Extraction and Separation of Photosynthetic Pigments

Autor/inn/en	Katayama, Nobuyasu; Kanaizuka, Yasuhiro; Sudarmi, Rini; Yokohama, Yasutsugu
Titel	An Improved Method for Extraction and Separation of Photosynthetic Pigments
Quelle	In: Journal of Biological Education, 37 (2003) 4, S.186-189 (4 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
Schlagwörter	High Schools; Chemistry; Laboratories; Botany; Foreign Countries; Secondary School Science; Science Experiments; Teaching Methods; Science Instruction; Scientific Methodology; Biology; Science Education; Plants (Botany); Color; Light; Japan + Suchen Sie Ihr Suchwort? High school; Oberschule; Chemie; Laboratory; Laboratorium; Botanik; Ausland; Teaching method; Lehrmethode; Unterrichtsmethode; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biologie; Naturwissenschaftliche Bildung; Pflanze; Colour; Farbbezeichnung; Farbe; Licht
Abstract	The method for extracting and separating hydrophobic photosynthetic pigments proposed by Katayama "et al." ("Japanese Journal of Phycology," 42, 71-77, 1994) has been improved to introduce it to student laboratories at the senior high school level. Silica gel powder was used for removing water from fresh materials prior to extracting pigments by a mixture of organic solvents that was also used for chromatographic separation of the pigments. A small silica gel thin-layer plate or a paper strip was used for separating the pigments. The improved method may be applicable to all kinds of plant materials including algae, is easier than most other methods, and can lead to more successful results in separating these pigments by both thin-layer chromatography and paper chromatography. The method has been carried out in student laboratories in some senior high schools and universities in Japan. The results indicate that this laboratory exercise is effective for students to recognise the unity and diversity of plants. Therefore, this laboratory seems to be useful for teaching plant systematics as well as for teaching photosynthesis. (Contains 1 figure and 1 online reference.) (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10