A Role for Synapsin in Associative Learning: The "Drosophila" Larva as a Study Case

Autor/inn/en	Michels, Birgit; Diegelmann, Soren; Tanimoto, Hiromu; Schwenkert, Isabell; Buchner, Erich; Gerber, Bertram
Titel	A Role for Synapsin in Associative Learning: The "Drosophila" Larva as a Study Case
Quelle	In: Learning & Memory, 12 (2005) 3, S.224-231 (8 Seiten) PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1072-0502
DOI	10.1101/lm.92805
Schlagwörter	Animals; Associative Learning; Role; Neurology; Genetics; Molecular Biology; Sensory Experience; Psychomotor Skills; Stimuli; Case Studies + Suchen Sie Ihr Suchwort? Animal; Tier; Tiere; Rollen; Neurologie; Humangenetik; Molekularbiologie; Sinnerfahrung; Psychomotorische Aktivität; Anreizsystem; Case study; Fallstudie; Case Study
Abstract	Synapsins are evolutionarily conserved, highly abundant vesicular phosphoproteins in presynaptic terminals. They are thought to regulate the recruitment of synaptic vesicles from the reserve pool to the readily-releasable pool, in particular when vesicle release is to be maintained at high spiking rates. As regulation of transmitter release is a prerequisite for synaptic plasticity, we use the fruit fly "Drosophila" to ask whether Synapsin has a role in behavioral plasticity as well; in fruit flies, Synapsin is encoded by a single gene ("syn"). We tackled this question for associative olfactory learning in larval "Drosophila" by using the deletion mutant "syn[superscript 97CS]," which had been backcrossed to the Canton-S wild-type strain (CS) for 13 generations. We provide a molecular account of the genomic status of "syn[superscript 97CS]" by PCR and show the absence of gene product on Western blots and nerve-muscle preparations. We found that olfactory associative learning in "syn[superscript 97CS]" larvae is reduced to [Similar to]50% of wild-type CS levels; however, responsiveness to the to-be-associated stimuli and motor performance in untrained animals are normal. In addition, we introduce two novel behavioral control procedures to test stimulus responsiveness and motor performance after "sham training." Wild-type CS and "syn[superscript 97CS]" perform indistinguishably also in these tests. Thus, larval "Drosophila" can be used as a case study for a role of Synapsin in associative learning. (Contains 4 figures.) (Author).
Anmerkungen	Cold Spring Harbor Laboratory Press. 500 Sunnyside Boulevard, Woodbury, NY 11797-2924. Tel: 800-843-4388; 516-367-8800; Fax: 516-422-4097; e-mail: cshpres@cshl.edu; Web site: http://www.learnmem.org/
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10