Characterization of Proteinous Coagulant in "Moringa" Tree Seeds for Water Purification: Stepwise Laboratory Exercise for High-School Students

Autor/inn/en	Oyama, Hiroshi; Nabeshima, Yuka; Morimoto, Koichi; Sugimura, Yukio
Titel	Characterization of Proteinous Coagulant in "Moringa" Tree Seeds for Water Purification: Stepwise Laboratory Exercise for High-School Students
Quelle	In: Biochemistry and Molecular Biology Education, 51 (2023) 5, S.588-599 (12 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Oyama, Hiroshi)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21763
Schlagwörter	Science Education; Science Laboratories; High School Students; Hands on Science; Pollution; Water; Intervention; Biochemistry; Scientific Concepts; Water Quality; Secondary School Science + Suchen Sie Ihr Suchwort? Naturwissenschaftliche Bildung; High school; High schools; Student; Students; Oberschule; Schüler; Schülerin; Studentin; Schadstoffbelastung; Wasser; Biochemie; Wasserqualität
Abstract	Coagulation is an important process in the context of water purification; and the seed protein of the moringa tree ("Moringa oleifera") is a remarkably effective coagulant. The laboratory course described here is designed to provide high-school students with a stepwise, hands-on experience in investigating the protein-rich coagulant found in "Moringa" seeds. First, the seed powder was applied to model polluted water containing fine clay, food dyes, copper sulfate, and bacteria. This treatment changed the polluted water into clear water via coagulation; all students were convinced that the coagulation-inducing agent was a thermostable cationic protein. Finally, basic biochemical techniques (e.g., chromatographic separation and electrophoresis) were used to show that the target coagulant is a dimeric protein composed of 6.5 and 4.5 kDa subunits. Overall, this made it possible for the students to gain a deeper understanding (more comprehensive than the information taught in formal classes) of protein structure and its real-world implications. This stepwise exercise can be applied to research-based learning programs in high school, as it is an effective learning tool. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01