Characterizing Biological Macromolecules with Attenuated Total Reflectance--Fourier Transform Infrared Spectroscopy Provides Hands-On Spectroscopy Experiences for Undergraduates

Autor/inn/en	Helburn, Robin; Nolan, Kathleen
Titel	Characterizing Biological Macromolecules with Attenuated Total Reflectance--Fourier Transform Infrared Spectroscopy Provides Hands-On Spectroscopy Experiences for Undergraduates
Quelle	In: Biochemistry and Molecular Biology Education, 50 (2022) 4, S.381-392 (12 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Helburn, Robin)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21619
Schlagwörter	Biology; Hands on Science; Spectroscopy; Learning Experience; Undergraduate Students; New York (New York) + Suchen Sie Ihr Suchwort? Biologie; Spektroskopie; Lernerfahrung
Abstract	Infrared (IR) spectra of biologically derived materials display distinct absorption bands correlating to individual macromolecules: protein, polysaccharide, lipid, and nucleic acids. A series of experiments aimed at teaching qualitative bioorganic spectroscopy using attenuated total reflectance (ATR) Fourier transform infrared (IR) with biological polymers as samples is proposed. Labs targeting 1st and 4th year undergraduate students at St. Francis College are being developed. During 2014 [right arrow] 2017, an integrated biology/chemistry exercise featuring elementary spectroscopy as an addition to an existing lab on light microscopy was administered to three sections of a 1st year general biology course. Students were taught the concept of a spectral fingerprint and to identify carbohydrate and protein based materials by looking for key vibrational bands. The success of that effort as determined by the results of an assessment quiz became the motivation for developing an advanced 4th year exercise involving four macromolecules. In a trial lab (Spring 2019) students gathered reference spectra from materials homogeneous in a single biopolymer followed by spectra of whole tissues which they were expected to fully characterize. Assessment data suggest that 1st year students benefited most from the experience. A detailed discussion of reference and sample spectra (as obtained by students) and relevant bond vibrations along with suggestions for instructors are presented. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01