Sodium Carbonate versus Borate Buffer for Lactase Quenching, Laboratory Work

Autor/inn/en	Ramos-de-la-Peña, Ana Mayela; Mercado-Valenzo, Oscar Mario; Clorio-Carrillo, Jorge Abraham; López-Incio, Jazmin Dessiret; Monroy-Borrego, Andrea Gabriela; Marrero-Bretado, Mariana Monserrat; González-Valdez, José; Aguilar, Oscar
Titel	Sodium Carbonate versus Borate Buffer for Lactase Quenching, Laboratory Work
Quelle	In: Biochemistry and Molecular Biology Education, 49 (2021) 6, S.935-941 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Ramos-de-la-Peña, Ana Mayela) ORCID (González-Valdez, José) ORCID (Aguilar, Oscar)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21567
Schlagwörter	Science Activities; Undergraduate Students; Advanced Courses; College Science; Science Instruction; Science Laboratories; Biochemistry + Suchen Sie Ihr Suchwort? Fortgeschrittenenunterricht; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Biochemie
Abstract	A laboratory exercise for undergraduate advanced students of enzymology and biocatalysis is presented. Since enzyme assays can be quenched or continuous, this experiment compares the performance of two quenching agents for lactase, in a continuous setup. Enzymatic activity of [beta]-galactosidase ("Aspergillus oryzae") was determined based on the release of 4-nitrophenol from 4-nitrophenyl [beta]-D-galactopyranoside using a microplate reader. Sodium carbonate and borate buffer were tested as quenching agents, and experimental control was the unstopped assay. Based on released 4-nitrophenol, enzyme activity, and rate constant k, the students could assess the performance of each termination agent. The experiment promotes disciplinary and transversal competencies, including research-based learning, critical thinking, and introduce the students to high-throughput techniques that are common in the research and development environment. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01