A Virtual ELISA to Quantitate COVID-19 Antibodies in Patient Serum

Autor/inn/en	Simpson, Kevin; Jakubowski, Henry V.
Titel	A Virtual ELISA to Quantitate COVID-19 Antibodies in Patient Serum
Quelle	In: Biochemistry and Molecular Biology Education, 48 (2020) 5, S.467-468 (2 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Jakubowski, Henry V.)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21403
Schlagwörter	Biochemistry; Science Laboratories; COVID-19; Patients; Mathematical Models; Laboratory Experiments + Suchen Sie Ihr Suchwort? Biochemie; Patient; Mathematical model; Mathematisches Modell; Laboratory work; Laborarbeit
Abstract	Enzyme-linked immunosorbent assays (ELISAs) are used widely in biotechnology, pharmaceutical, and clinical medicine labs. At the same time, they appear to be underrepresented in chemistry and biochemistry curricula, even though their sensitivity, selectivity, and ease of use would argue for their widespread use. We describe here an online ELISA activity suitable for stand-alone use or in conjunction with an actual wet lab ELISA. Specifically, we offer real and mock data for a hypothetical ELISA to detect plasma antibodies to COVID-19 in infected patients who have had the disease. Much of the activity focuses on chemical and mathematical models to fit ELISA or any macromolecule/ligand binding data, a skill that addresses perhaps the most relevant and difficult learning goal of an ELISA experiment. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01