Fabrication of a Low-Cost On-Stage Cell Incubator with Full Automation

Autor/inn/en	Ragazzini, Gregorio; Mescola, Andrea; Corsi, Lorenzo; Alessandrini, Andrea
Titel	Fabrication of a Low-Cost On-Stage Cell Incubator with Full Automation
Quelle	In: Journal of Biological Education, 53 (2019) 2, S.165-173 (9 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Ragazzini, Gregorio) ORCID (Corsi, Lorenzo) ORCID (Alessandrini, Andrea)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	0021-9266
DOI	10.1080/00219266.2018.1451772
Schlagwörter	Cytology; Science Instruction; Laboratory Equipment; Measurement; Computer Software; Optics; Laboratory Experiments; Biology + Suchen Sie Ihr Suchwort? Zytologie; Teaching of science; Science education; Natural sciences Lessons; Naturwissenschaftlicher Unterricht; Laborausstattung; Messverfahren; Optik; Laboratory work; Laborarbeit; Biologie
Abstract	In recent years the possibility of observing by microscopy the dynamic activity of living cells has been largely pursued. We have developed a low-cost (~ 260 euros) on-stage cell incubator for inverted optical microscopes. This device allows to keep cells in good conditions for their survival and proliferation. The device is based on the use of the Arduino microprocessor interfaced with LabView. It can be connected to a computer via USB port allowing to monitor and register all the useful parameters of the measurements: temperature, CO2 concentration and relative humidity. It consists of a closed metallic and plastic (PMMA) chassis which provides optical transparency to the petri dish in order to use interference contrast imaging techniques. The system exploits also a second Arduino microprocessor to perform autofocus of the images and to automatically acquire images at defined time intervals. Cell biology laboratories could easily construct this device to allow also students to follow dynamic processes of living cells and to practice with the DIY (Do-It-Yourself) approach to biology. At the same time, students could become familiar with the use of low-cost microprocessors like Arduino. (As Provided).
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Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2020/1/01