A Molecular Genetics Laboratory Course Applying Bioinformatics and Cell Biology in the Context of Original Research

Autor/inn/en	Brame, Cynthia J.; Pruitt, Wendy M.; Robinson, Lucy C.
Titel	A Molecular Genetics Laboratory Course Applying Bioinformatics and Cell Biology in the Context of Original Research
Quelle	In: CBE - Life Sciences Education, 7 (2008) 4, S.410-421 (12 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1931-7913
DOI	10.1187/cbe.08-07-0036
Schlagwörter	Student Research; Computer Uses in Education; Educational Technology; Student Reaction; Student Interests; Science Interests; Genetics; Science Laboratories; Cytology; Molecular Biology; College Science + Suchen Sie Ihr Suchwort? Studentenforschung; Computernutzung; Unterrichtsmedien; Schülerkritik; Studieninteresse; Humangenetik; Zytologie; Molekularbiologie
Abstract	Research based laboratory courses have been shown to stimulate student interest in science and to improve scientific skills. We describe here a project developed for a semester-long research-based laboratory course that accompanies a genetics lecture course. The project was designed to allow students to become familiar with the use of bioinformatics tools and molecular biology and genetic approaches while carrying out original research. Students were required to present their hypotheses, experiments, and results in a comprehensive lab report. The lab project concerned the yeast casein kinase 1 (CK1) protein kinase Yck2. CK1 protein kinases are present in all organisms and are well conserved in primary structure. These enzymes display sequence features that differ from other protein kinase subfamilies. Students identified such sequences within the CK1 subfamily, chose a sequence to analyze, used available structural data to determine possible functions for their sequences, and designed mutations within the sequences. After generating the mutant alleles, these were expressed in yeast and tested for function by using two growth assays. The student response to the project was positive, both in terms of knowledge and skills increases and interest in research, and several students are continuing the analysis of mutant alleles as summer projects. (Contains 5 figures and 4 tables.) (As Provided).
Anmerkungen	American Society for Cell Biology. 8120 Woodmont Avenue Suite 750, Bethesda, MD 20814-2762. Tel: 301-347-9300; Fax: 301-347-9310; E-mail: ascbinfo@ascb.org; Website: http://www.ascb.org
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2017/4/10