Literaturnachweis - Detailanzeige
| Autor/inn/en | Taipa, M. A^ngela; Azevedo, Ana M.; Grilo, Anto´nio L.; Couto, Pedro T.; Ferreira, Filipe A. G.; Fortuna, Ana R. M.; Pinto, Ine^s F.; Santos, Rafael M.; Santos, Susana B. |
|---|---|
| Titel | Student Collaboration in a Series of Integrated Experiments to Study Enzyme Reactor Modeling with Immobilized Cell-Based Invertase |
| Quelle | In: Journal of Chemical Education, 92 (2015) 7, S.1238-1243 (6 Seiten)Infoseite zur Zeitschrift
PDF als Volltext |
| Sprache | englisch |
| Dokumenttyp | gedruckt; online; Zeitschriftenaufsatz |
| ISSN | 0021-9584 |
| DOI | 10.1021/ed500842p |
| Schlagwörter | Cooperative Learning; Science Experiments; College Science; Undergraduate Study; Biotechnology; Biochemistry; Chemical Engineering; Science Laboratories; Laboratory Experiments; Scientific Concepts; Teaching Methods; Kinetics; Foreign Countries; Science Instruction; Portugal |
| Abstract | An integrative laboratory study addressing fundamentals of enzyme catalysis and their application to reactors operation and modeling is presented. Invertase, a ß-fructofuranosidase that catalyses the hydrolysis of sucrose, is used as the model enzyme at optimal conditions (pH 4.5 and 45 °C). The experimental work involves 3 h of laboratory time for each student per week over four weeks. Students, organized in laboratory sessions of three groups with 3 or 4 students per group, work in a collaborative manner to obtain a set of replicates of initial reaction rate for different substrate concentrations, using both free and calcium alginate-immobilized "Saccharomyces bayanus" cells containing biologically active invertase. The results are shared by all of the groups for statistical data treatment, calculation of Michaelis-Menten kinetic parameters, and modeling of three types of classical reactors, a Continuous Stirred Tank Reactor (CSTR), a Plug Flow Reactor (PFR), and a Fluidized Bed Reactor (FBR), operating in continuous mode with immobilized invertase. For each reactor, the experimental conversion profile as a function of the feed flow rate is compared to predicted profiles based upon the kinetic parameters obtained. (As Provided). |
| Anmerkungen | Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc |
| Erfasst von | ERIC (Education Resources Information Center), Washington, DC |
| Update | 2020/1/01 |
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