A Simple Colorimetric Experiment Using Mammalian Cell Culture to Study Metabolism

Autor/inn/en	Sharma, Kanika; Fizet, Kiel J.; Montgomery, Kathlyene R.; Smeltzer, Nathan A.; Sikorski, Madison H.; Brown, Kennedy G.; Beyke, Brandon J.; Burkhart, Ryan C.; Lynn, Abigail N.; Grandinetti, Giovanna
Titel	A Simple Colorimetric Experiment Using Mammalian Cell Culture to Study Metabolism
Quelle	In: Biochemistry and Molecular Biology Education, 49 (2021) 2, S.271-277 (7 Seiten)Infoseite zur Zeitschrift PDF als Volltext Verfügbarkeit
Zusatzinformation	ORCID (Grandinetti, Giovanna)
Sprache	englisch
Dokumenttyp	gedruckt; online; Zeitschriftenaufsatz
ISSN	1470-8175
DOI	10.1002/bmb.21457
Schlagwörter	Biochemistry; Cytology; Metabolism; Science Laboratories; Laboratory Procedures; Undergraduate Students; Laboratory Experiments; Pharmacology; Animals + Suchen Sie Ihr Suchwort? Biochemie; Zytologie; Stoffwechsel; Laboruntersuchung; Laboratory work; Laborarbeit; Arzneikunde; Animal; Tier; Tiere
Abstract	The goal of this laboratory exercise is to give upper-level undergraduate students an introduction to sterile technique in mammalian cell culture and metabolism. The experiment can be completed within a 3-h lab period and can be performed either in conjunction with other biochemistry/metabolism experiments or used as a stand-alone experiment. In this experiment, students are tasked with relating the acidification of cell culture medium to metabolism in order to elucidate the mechanism of action for a compound. Students can relate their experimental results to topics covered on glycolysis and oxidative phosphorylation in upper-level biochemistry classes as well as gain valuable experience relating metabolism to drug discovery. (As Provided).
Anmerkungen	Wiley. Available from: John Wiley & Sons, Inc. 111 River Street, Hoboken, NJ 07030. Tel: 800-835-6770; e-mail: cs-journals@wiley.com; Web site: https://www.wiley.com/en-us
Erfasst von	ERIC (Education Resources Information Center), Washington, DC
Update	2024/1/01